ABSTRACT
Objective To investigate the serum levels of CD147 and MMP-2 and the relationship between these proteins and colorectal tumor differentiation, Dukes stage. Methods Serum levels of CD147 and MMP-2 in 44 patients with colorectal carcinoma,28 polyp intestinal patients and 36 normal subjects were measured by ELISA. Results Serum levels of CD147(103.59±40.74pg/ml)and MMP-2(14.92±2.02ng/ml)in the cancer patients were significantly higher than those of the polyp intestinal(66.27±16.91;10:96±1.71)and normal control(63.84±18.71;10.49±1.61)(P＜0.05).The levels were associated with tumor differentiation and Dukes stage. Patients with poor differentiation had significantly higher levels of MMP-2 than those of patients with well differentiation. And Dukes C and D stage tumors had significantly higher levels of CD147 than Dukes A and B stage tumors(P＜0.05).The levels of CD147 and MMP-2 declined remarkably after one month of radical operation. Conversely, it decreased illegibly after palliative operation. The serum levels of CD147were positively correlated with MMP-2 in patients with colorectal cancer. Conclusion Elevated CD147 and MMP-2 serum levels are associated with tumor differentiation, invasion, metastasis. Dynamic alterations of serum CD147 and MMP-2 may be used as the indicators of diagnosis, the choice of operative method and the judgment of prognosis in patients with colorectal cancer.
ABSTRACT
Effects of squalene, algae (Enteromorpha prolifera), laver (Prophyra hai-tanensis) and cauliflower on serum total cholesterol (T-C), high density li-poprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and very low density lipoprotein cholesterol (VLDL-C) were separatly observed in Wistar male albino rats fed on a diet containing 0.5% cholesterol,5% lard and 0.5% bile salt. Squalene was supplemented at 5% of the diet, and algae, laver, cauliflower at 10% of the diet.After the rats had been fed on the diet described above for 30 days, serum T-C, HDL-C, LDL-C and VLDL-C levels were determined. The results showed that the rats fed on a diet containing 5% squalene or 10% algae were significantly lower in serum T-C, HDL-C and VLDL-C but higher in serum HDL-C than those of the controls, and the rats fed on a diet containing 10% laver or 10% cauliflower were significantly lower in serum T-C than that of the controls.